The Antibacterial Property of Nigella sativa (Black seed) Oil Against Gram-positive and Gramnegative Bacteria
https://doi.org/10.24017/science.2021.2.15
Abstract views: 1463 / PDF downloads: 931Abstract
Antimicrobial resistance is one of the most challenges ? that face the world today, and it is heading toward the post-antibiotic era. Traditionally, herbal extracts, medicinal plant oil extracts, and probiotics have been used as an alternative to antibiotics due to bacterial resistance and drug side effects. This ?work is carried out to evaluate the antimicrobial efficacy of Nigella sativa oil extracts ?against common resistant bacteria, including Methicillin-resistant staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. The black seed was purchased from the traditional herbal medicine market, Hot extraction of the oil was performed. The bacteria were obtained from a microbiology company and from clinical samples at the Shar hospital in Sulaimani Provence. The bacteria were gram-negative (Salmonella enterica, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive (methicillin-resistant Staphylococcus aureus (MRSA), Bacillus subtilis, and Bacillus cereus). The agar ?diffusion well technique and minimum inhibitory concentration (MIC) were used. The black seed oil components were analyzed by the Gas Chromatograph mass spectrum. The results revealed that the oil of Nigella sativa shows a ?significant effect on Gram-positive bacteria, including MRSA, which is known as a multidrug resistance bacterium. ? Bacillus subtilis was more sensitive than other strains, and the significant antibacterial effect of the extracted was observed against Escherichia coil, while it has no significant effect on Pseudomonas aeruginosa and Salmonella enterica.? Determination of Nigella sativa oil minimum inhibitory concentration (MIC) for Gram-positive bacteria is 100 ml for Staphylococcus aureus and Bacillus subtilis, and the MIC was 200 ml and 400 ml for Bacillus cereus and MRSA respectively. This study concludes that oil extract of Nigella sativa is a ?good natural antimicrobial, it can be used against ?MRSA and other Gram-positive bacteria.
Keywords:
Nigella sativa, antimicrobial effect, antibiotic, multi-drug-resistant Staphylococcus aureus.
References
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https://doi.org/10.17221/2948-VETMED
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https://doi.org/10.3748/wjg.v11.i42.6684
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https://doi.org/10.1016/S0034-5288(02)00108-X
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https://doi.org/10.2174/1389201019666181113122009
[20] M. O. Al Turkmani, F. Karabet, L. Mokrani, and C. Soukkarieh, "Chemical composition and in vitro antioxidant activities of essential oil from Nigella sativa L. seeds cultivated in Syria," International Journal of ChemTech Research, vol. 8, no. 10, pp. 76-82, 2015.
[21] B. R. Othman et al., "Occurrence of methicillin-resistant Staphylococcus aureus in raw shellfish at retail markets in Malaysia and antibacterial efficacies of black seed (Nigella sativa) oil against MRSA," Food Control, vol. 90, pp. 324-331, 2018.
https://doi.org/10.1016/j.foodcont.2018.02.045
[22] L. B. Emeka, P. M. Emeka, and T. M. Khan, "Antimicrobial activity of Nigella sativa L. seed oil against multi-drug resistant Staphylococcus aureus isolated from diabetic wounds," Pakistan journal of pharmaceutical sciences, vol. 28, no. 6, 2015.
[23] S. Javed, A. A. Shahid, M. S. Haider, A. Umeera, R. Ahmad, and S. Mushtaq, "Nutritional, phytochemical potential and pharmacological evaluation of Nigella Sativa (Kalonji) and Trachyspermum Ammi (Ajwain)," Journal of Medicinal Plants Research, vol. 6, no. 5, pp. 768-775, 2012.
https://doi.org/10.5897/JMPR11.1341
[24] H. Zuridah, A. Fairuz, A. Zakri, and M. Rahim, "In vitro antibacterial activity of Nigella sativa against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Bacillus cereus," Asian journal of plant sciences, vol. 7, no. 3, pp. 331-333, 2008.
https://doi.org/10.3923/ajps.2008.331.333
[25] N. A. Shova, "Comparative study on the antibacterial activities of neem oil, mustard oil and black seed oil against Staphylococcus aureus, Klebsiella pneumoniae, Bacillus cereus, Salmonella typhi and Pseudomonas aeruginosa," BRAC Univeristy, 2017.
[26] M. Safhi, M. Alam, S. Sivakumar, J. Aamena, A. Tairiq, and I. Fakhrul, "Antibacterial spectrum of black seed oil against selected human pathogenic bacteria," J Pharm Res, vol. 8, pp. 1726-8, 2014.
[27] A. Ferdous, S. Islam, M. Ahsan, C. Hasan, and Z. Ahmed, "In vitro antibacterial activity of the volatile oil of Nigella sativa seeds against multiple drug?resistant isolates of Shigella spp. and isolates of Vibrio cholerae and Escherichia coli," Phytotherapy Research, vol. 6, no. 3, pp. 137-140, 1992.
https://doi.org/10.1002/ptr.2650060307
[28] K. Callahan, N. Beck, E. Duffield, G. Shin, and J. Meschke, "Inactivation of methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VRE) on various environmental surfaces by mist application of a stabilized chlorine dioxide and quaternary ammonium compound-based disinfectant," Journal of occupational and environmental hygiene, vol. 7, no. 9, pp. 529- 534, 2010.
https://doi.org/10.1080/15459624.2010.487806
[29] K. Srinivasan, "Cumin (Cuminum cyminum) and black cumin (Nigella sativa) seeds: traditional uses, chemical constituents, and nutraceutical effects," Food quality and safety, vol. 2, no. 1, pp. 1-16, 2018.
https://doi.org/10.1093/fqsafe/fyx031
[30] J.-P. Rauha, J.-L. Wolfender, J.-P. Salminen, K. Pihlaja, K. Hostettmann, and H. Vuorela, "Characterization of the polyphenolic composition of purple loosestrife (Lythrum salicaria)," Zeitschrift für Naturforschung C, vol. 56, no. 1-2, pp. 13-20, 2001.
https://doi.org/10.1515/znc-2001-1-203
[31] M. S. Ahamad Bustamam et al., "Stability study of Algerian Nigella sativa seeds stored under different conditions," Journal of analytical methods in chemistry, vol. 2017, 2017.
https://doi.org/10.1155/2017/7891434
[2] I. Yaman, A.S. Durmus, S. Ceribasi, and M. Yaman, "Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats," Veterinarni Medicina, vol. 55, no. 12, pp. 619-624, 2010.
https://doi.org/10.17221/2948-VETMED
[3] H. Hosseinzadeh, S. Parvardeh, M. N. Asl, H. R. Sadeghnia, and T. Ziaee, "Effect of thymoquinone and Nigella sativa seeds oil on lipid peroxidation level during global cerebral ischemia-reperfusion injury in rat hippocampus," Phytomedicine, vol. 14, no. 9, pp. 621-627, 2007.
https://doi.org/10.1016/j.phymed.2006.12.005
[4] M. A. Mansour, M. N. Nagi, A. S. El?Khatib, and A. M. Al?Bekairi, "Effects of thymoquinone on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase in different tissues of mice: a possible mechanism of action," Cell biochemistry and function, vol. 20, no. 2, pp. 143-151, 2002.
https://doi.org/10.1002/cbf.968
[5] J. Ahmad, R. A. Khan, and M. A. Malik, "A study of Nigella sativa oil in the management of wheeze associated lower respiratory tract illness in children," African journal of pharmacy and pharmacology, vol. 4, no. 7, pp. 436-439, 2010.
[6] A. M. Alsamarai, M. A. Satar, and A. H. A. Alobaidi, "Evaluation of therapeutic efficacy of Nigella sativa (Black Seed) for treatment of allergic rhinitis," in Allergic Rhinitis: InTech, 2012.
[7] H. I??k, A. Çevikba?, U.S. Gürer, B. K?ran, Y. Üresin, P. Rayaman, E. Rayaman, B. Gürbüz, and S. Büyüköztürk, "Potential adjuvant effects of Nigella sativa seeds to improve specific immunotherapy in allergic rhinitis patients," Medical Principles and Practice, vol. 19, no. 3, pp. 206-211, 2010.
https://doi.org/10.1159/000285289
[8] T. A. Gheita and S. A. Kenawy, "Effectiveness of Nigella sativa oil in the management of rheumatoid arthritis patients: a placebo controlled study," Phytotherapy research, vol. 26, no. 8, pp. 1246-1248, 2012.
https://doi.org/10.1002/ptr.3679
[9] S. Kheirouri, V. Hadi, and M. Alizadeh, "Effects of Nigella sativa oil extract on inflammatory cytokine response and oxidative stress status in patients with rheumatoid arthritis; a randomized, double-blind, placebo-controlled clinical trial," 2014.
[10] S. Gharby, H. Harhar, D. Guillaume, A. Roudani, S. Boulbaroud, M. Ibrahimi, M. Ahmad, S. Sultana, T.B. Hadda, I. Chafchaouni-Moussaoui, Z. Charrouf., "Chemical investigation of Nigella sativa L. seed oil produced in Morocco," Journal of the Saudi Society of Agricultural Sciences, vol. 14, no. 2, pp. 172-177, 2015.
https://doi.org/10.1016/j.jssas.2013.12.001
[11] A. R. Khan and K. Kour, "Wide spectrum antibacterial activity of Nigella Sativa L. seeds."
[12] A. A. Hama and S. A. Qadir, "The antimicrobial activity of pomegranate (Punica granatum) juice," International Journal of Scientific & Engineering Research, vol. 5, no. 10, pp. 796-798, 2014.
[13] S. M. Weli, S. I. Kareem, O. H. Shareef, G. Rauf, C. G. Raouf, and A. A. Hama, "The Effects of the Aqueous Extract of the Leaves of the Local Plant Eruca sativa on Lipid Profile and Some Minerals in the Blood of Male White Mice Eruca sativa" Iraqi Journal of Science vol. 62, no. 4, pp. 1088-1093, 2021.
https://doi.org/10.24996/ijs.2021.62.4.5
[14] M. Samadi, Z. Z. Abidin, R. Yunus, D. R. Awang Biak, H. Yoshida, and E. H. Lok, "Assessing the kinetic model of hydro-distillation and chemical composition of Aquilaria malaccensis leaves essential oil," Chinese J. Chem. Eng., vol. 25, no. 2, pp. 216-222, 2017.
https://doi.org/10.1016/j.cjche.2016.09.006
[15] L. B. Reller, M. Weinstein, J. H. Jorgensen, and M. J. Ferraro, "Antimicrobial susceptibility testing: a review of general principles and contemporary practices," Clinical infectious diseases, vol. 49, no. 11, pp. 1749-1755, 2009.
https://doi.org/10.1086/647952
[16] A. Bauer, "Antibiotic susceptibility testing by a standardized single disc method," Am J clin pathol, vol. 45, pp. 149-158, 1966.
https://doi.org/10.1093/ajcp/45.4_ts.493
[17] M. Kanter, O. Coskun, and M. Budancamanak, "Hepatoprotective effects of Nigella sativa L and Urtica dioica L on lipid peroxidation, antioxidant enzyme systems and liver enzymes in carbon tetrachloridetreated rats," World journal of gastroenterology: WJG, vol. 11, no. 42, p. 6684, 2005.
https://doi.org/10.3748/wjg.v11.i42.6684
[18] K. Fararh, Y. Atoji, Y. Shimizu, and T. Takewaki, "Isulinotropic properties of Nigella sativa oil in Streptozotocin plus Nicotinamide diabetic hamster," Research in veterinary science, vol. 73, no. 3, pp.279-282, 2002.
https://doi.org/10.1016/S0034-5288(02)00108-X
[19] M. A. Khan and H. Younus, "Thymoquinone shows the diverse therapeutic actions by modulating multiple cell signaling pathways: single drug for multiple targets," Current pharmaceutical biotechnology, vol. 19, no. 12, pp. 934-945, 2018.
https://doi.org/10.2174/1389201019666181113122009
[20] M. O. Al Turkmani, F. Karabet, L. Mokrani, and C. Soukkarieh, "Chemical composition and in vitro antioxidant activities of essential oil from Nigella sativa L. seeds cultivated in Syria," International Journal of ChemTech Research, vol. 8, no. 10, pp. 76-82, 2015.
[21] B. R. Othman et al., "Occurrence of methicillin-resistant Staphylococcus aureus in raw shellfish at retail markets in Malaysia and antibacterial efficacies of black seed (Nigella sativa) oil against MRSA," Food Control, vol. 90, pp. 324-331, 2018.
https://doi.org/10.1016/j.foodcont.2018.02.045
[22] L. B. Emeka, P. M. Emeka, and T. M. Khan, "Antimicrobial activity of Nigella sativa L. seed oil against multi-drug resistant Staphylococcus aureus isolated from diabetic wounds," Pakistan journal of pharmaceutical sciences, vol. 28, no. 6, 2015.
[23] S. Javed, A. A. Shahid, M. S. Haider, A. Umeera, R. Ahmad, and S. Mushtaq, "Nutritional, phytochemical potential and pharmacological evaluation of Nigella Sativa (Kalonji) and Trachyspermum Ammi (Ajwain)," Journal of Medicinal Plants Research, vol. 6, no. 5, pp. 768-775, 2012.
https://doi.org/10.5897/JMPR11.1341
[24] H. Zuridah, A. Fairuz, A. Zakri, and M. Rahim, "In vitro antibacterial activity of Nigella sativa against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Bacillus cereus," Asian journal of plant sciences, vol. 7, no. 3, pp. 331-333, 2008.
https://doi.org/10.3923/ajps.2008.331.333
[25] N. A. Shova, "Comparative study on the antibacterial activities of neem oil, mustard oil and black seed oil against Staphylococcus aureus, Klebsiella pneumoniae, Bacillus cereus, Salmonella typhi and Pseudomonas aeruginosa," BRAC Univeristy, 2017.
[26] M. Safhi, M. Alam, S. Sivakumar, J. Aamena, A. Tairiq, and I. Fakhrul, "Antibacterial spectrum of black seed oil against selected human pathogenic bacteria," J Pharm Res, vol. 8, pp. 1726-8, 2014.
[27] A. Ferdous, S. Islam, M. Ahsan, C. Hasan, and Z. Ahmed, "In vitro antibacterial activity of the volatile oil of Nigella sativa seeds against multiple drug?resistant isolates of Shigella spp. and isolates of Vibrio cholerae and Escherichia coli," Phytotherapy Research, vol. 6, no. 3, pp. 137-140, 1992.
https://doi.org/10.1002/ptr.2650060307
[28] K. Callahan, N. Beck, E. Duffield, G. Shin, and J. Meschke, "Inactivation of methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium (VRE) on various environmental surfaces by mist application of a stabilized chlorine dioxide and quaternary ammonium compound-based disinfectant," Journal of occupational and environmental hygiene, vol. 7, no. 9, pp. 529- 534, 2010.
https://doi.org/10.1080/15459624.2010.487806
[29] K. Srinivasan, "Cumin (Cuminum cyminum) and black cumin (Nigella sativa) seeds: traditional uses, chemical constituents, and nutraceutical effects," Food quality and safety, vol. 2, no. 1, pp. 1-16, 2018.
https://doi.org/10.1093/fqsafe/fyx031
[30] J.-P. Rauha, J.-L. Wolfender, J.-P. Salminen, K. Pihlaja, K. Hostettmann, and H. Vuorela, "Characterization of the polyphenolic composition of purple loosestrife (Lythrum salicaria)," Zeitschrift für Naturforschung C, vol. 56, no. 1-2, pp. 13-20, 2001.
https://doi.org/10.1515/znc-2001-1-203
[31] M. S. Ahamad Bustamam et al., "Stability study of Algerian Nigella sativa seeds stored under different conditions," Journal of analytical methods in chemistry, vol. 2017, 2017.
https://doi.org/10.1155/2017/7891434
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[1]
S. A. Abdullah, T. F. M. Salih, A. A. Hama, S. I. Ali, and H. H. Hamaamin, “The Antibacterial Property of Nigella sativa (Black seed) Oil Against Gram-positive and Gramnegative Bacteria”, KJAR, vol. 6, no. 2, pp. 156–165, Jan. 2022, doi: 10.24017/science.2021.2.15.
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26-01-2022
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