Antibacterial Activity of Copper Nanoparticles Fabricate via Malva Sylvesteris Leaf Extract

https://doi.org/10.24017/science.2019.ICHMS.15

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Authors

  • Osama Ismail Haji Zebari Akre Directorate Education, Duhok General Directorate, Ministry of Education, Duhok, Iraq
  • Samie Yaseen Sharaf Zeebaree Dept. of Medical lab. Technology, Shekhan Technical College of Health, Duhok Polytechnic University, Duhok, Iraq
  • Aemn Yaseen Sharaf Zeebaree Dept. of Medical lab. Technology, Shekhan Technical College of Health, Duhok Polytechnic University, Duhok, Iraq
  • Hardan Ismail Haji Zebari Akre Directorate Education, Duhok General Directorate, Ministry of Education, Duhok, Iraq
  • Hadeel Ridha Abbas Maternity & Obstetric Department, Sulaimani Technical Institute, Sulaimani Polytechnic University, Sulaimani, Kurdistan Region, Iraq

Abstract

A Green reduction of copper ions Cu2+ has been achieved by one-step process and at room temperature utilizing Malva sylvesteris. The extract of Malva Sylvesteris leaf has been identified using qualitative tests to detect the bioactive compounds such as flavonoids, polyphenols, terpenoids and carbohydrates. Characterization of copper nanoparticles was diagnosed by Ultraviolet–visible spectroscopy that confirms the band characteristic for copper nanoparticles in the range of 200–700 nm and the role of Malva Selvesteris leaf extract biomolecules was confirmed by Fourier transform infrared spectroscopy. The crystal shape of nanoparticles was confirmed by X-ray diffraction (XRD) at average (20.96 nm) and the peaks correspond to the face centered cubic structure of cooper metal. Effective antiseptic activity of copper nanoparticles determined by measurement of inhibition zone showed against representative microorganism of bacteria (Gram-positive: Clostridium Staph.aureus;) and (Gram-negative: Escherichia col; Pseudomonas; Klebsiella

Keywords:

Fabrication, Nanoparticles, Malva sylvesteris , Microbial susceptibility

References

[1] M. B. Gawande et al., "Cu and Cu-Based Nanoparticles: Synthesis and Applications in Catalysis," Chem. Rev., vol. 116, no. 6, pp. 3722-3811, 2016.
https://doi.org/10.1021/acs.chemrev.5b00482
[2] A. Umer, S. Naveed, and N. Ramzan, "a Green Method for the Synthesis," pp. 197-203, 2014.
https://doi.org/10.1590/S1517-70762014000300002
[3] A. Y. Ghidan, T. M. Al-Antary, and A. M. Awwad, "Green synthesis of copper oxide nanoparticles using Punica granatum peels extract: Effect on green peach Aphid," Environ. Nanotechnology, Monit. Manag., vol. 6, pp. 95-98, 2016.
https://doi.org/10.1016/j.enmm.2016.08.002
[4] A. Nanda and M. Saravanan, "Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE," Nanomedicine Nanotechnology, Biol. Med., vol. 5, no. 4, pp. 452-456, 2009.
https://doi.org/10.1016/j.nano.2009.01.012
[5] K. M. El-Gamal, M. S. Hagrs, and H. S. Abulkhair, "Synthesis, characterization and antimicrobial evaluation of some novel quinoline derivatives bearing different heterocyclic moieties," Bull. Fac. Pharmacy, Cairo Univ., vol. 54, no. 2, pp. 263-273, 2016.
https://doi.org/10.1016/j.bfopcu.2016.08.002
[6] P. P. N. V. Kumar, U. Shameem, P. Kollu, R. L. Kalyani, and S. V. N. Pammi, "Green Synthesis of Copper Oxide Nanoparticles Using Aloe vera Leaf Extract and Its Antibacterial Activity Against Fish Bacterial Pathogens," Bionanoscience, vol. 5, no. 3, pp. 135-139, 2015.
https://doi.org/10.1007/s12668-015-0171-z
[7] M. Nasrollahzadeh, M. Atarod, and S. M. Sajadi, "Green synthesis of the Cu/Fe 3 O 4 nanoparticles using Morinda morindoides leaf aqueous extract: A highly efficient magnetically separable catalyst for the reduction of organic dyes in aqueous medium at room temperature," Appl. Surf. Sci., vol. 364, pp. 636-644, 2016.
https://doi.org/10.1016/j.apsusc.2015.12.209
[8] S. Shrivastava, T. Bera, A. Roy, G. Singh, P. Ramachandrarao, and D. Dash, "Characterization of enhanced antibacterial effects of novel silver nanoparticles," Nanotechnology, vol. 18, no. 22, 2007.
https://doi.org/10.1088/0957-4484/18/22/225103
[9] A. K. Chatterjee, R. Chakraborty, and T. Basu, "Mechanism of antibacterial activity of copper nanoparticles," Nanotechnology, vol. 25, no. 135101, pp. 1-12, 2014.
https://doi.org/10.1088/0957-4484/25/13/135101
[10] M. J. Hajipour, K. M. Fromm, A. A. Ashkarran, D. J. De Aberasturi, I. R. De Larramendi, and T. Rojo, "Antibacterial properties of nanoparticles," Trends Biotechnol., vol. 30, no. 10, pp. 499-511, 2012.
https://doi.org/10.1016/j.tibtech.2012.06.004
[11] J. Kim, H. Cho, S. Ryu, and M. Choi, "Effects of Metal Ions on the Activity of Protein Tyrosine Phosphatase VHR?: Highly Potent and Reversible Oxidative Inactivation by Cu 2 ? Ion," Arch. Biochem. Biophys., vol. 382, no. 1, pp. 72-80, 2000.
https://doi.org/10.1006/abbi.2000.1996

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How to Cite

[1]
O. Ismail Haji Zebari, S. Yaseen Sharaf Zeebaree, A. Yaseen Sharaf Zeebaree, H. Ismail Haji Zebari, and H. Ridha Abbas, “Antibacterial Activity of Copper Nanoparticles Fabricate via Malva Sylvesteris Leaf Extract”, KJAR, vol. 4, no. 3, pp. 146–156, Aug. 2019, doi: 10.24017/science.2019.ICHMS.15.

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Published

21-08-2019